First Author: Subramanian,Senying,Verónica,Lluch‐Senar,Puzi,Tianlong,Bai,Sanathoi,Gurumayum,Balakrishnan,Subramanian,Liting,Mengwan,Sicheng,Senying,Lai,Balakrishnan,Subramanian,Na,Sicheng,Dai,Yin-Ying,Members,Bioinformatics,of,Institute,Swiss,SIB,Huangkai,Maria,Lloréns-Rico,Verónica,Maria,Gerald,Schönknecht,Lluch‐Senar,Huangkai,Trachana,Guang-Zhong,Kalliopi,Trachana,He,Puzi,Sicheng,Weiwei,Yus,Lin,Zilong,He,L,Yu,Yu,Weiwei,Jiang,Jianzhong,Huang,Yu,Yus,Chen,Die,Yuan,Na,Jian,Gao,Wu,Zhang,Xue,Lu,L,Wei-Hua,Thomas,Bock,Wei-Hua,Ge,Lili,Hao,Yu,Weiwei,Wang,Song,Xiaomeng,Ge,Guanting,Guell,Sebastian,Kuhner,Wei-Hua,Chen,Eva,Yus,Marc,Guell,Sebastian,Kuhner

Co-author: Wei-Hua,Chen,Zhang,L,Na,Gao,Wang,Wei-Hua,Chen,Puzi,Jiang,Wei-Hua,Chen,Zhang,L,Na,Gao,Wang,He,L,Hao,Puzi,Jiang,Wei-Hua,Chen,Zhang,Balakrishnan,Subramanian,Wei-Hua,Chen,Zhang,Chen,Zhang,Wei-Hua,Chen,Sicheng,Wu,Senying,Lai,Wei-Hua,Chen,Xue,Hao,Wu,Wei-Hua,Chen,Xue,Die,Zhang,Chen,Zhang,Chen,Die,Dai,Senying,Lai,Wang,Wei-Hua,Chen,Gao,Wei-Hua,Chen,L,Na,Gao,Sicheng,Wu,Wang,Wei-Hua,Chen,Wang,Chen,Wei-Hua,Chen,Hao,Wei-Hua,Chen,Zhang,Zhang,Wei-Hua,Chen,Lin,Zhang,Wei-Hua,Chen,Guanting,Lu,Chen,Wang,Wu,Wei-Hua,Chen,Jian,Ge,Gao,Wu,Wang,Zhang,Chen,Guanting,Lu,Wei-Hua,Chen,Gao,Huangkai,Zhang,Guanting,Lu,Wei-Hua,Chen,Eva,Yus,L,Eva,Yus,Chen,L,Wei-Hua,Chen,Wei-Hua,Chen,Chen,Marc,Wei-Hua,Chen,Gao,Trachana,Wei-Hua,Chen,Wu,Wei-Hua,Chen,Yu,Xiaomeng,Ge,Yu,Weiwei,Wang,Song,Chen,Chen,Zhang,Jiang,Chen,Guanting,Wang,Lin,Wu,He,Lin,Wang,Yu,Xiaomeng,Ge,Chen,Marc,Guell,Wei-Hua,Chen,Sebastian,Kuhner,Wei-Hua,Chen,Eva,Wei-Hua,Chen,Puzi,Jiang,Wei-Hua,Chen,Zhang,L,Na,Gao,Wang,He,L,Hao,Puzi,Jiang,Wei-Hua,Chen,Zhang,Balakrishnan,Subramanian,Wei-Hua,Chen,Zhang,Chen,Zhang,Wei-Hua,Chen,Sicheng,Wu,Senying,Lai,Wei-Hua,Chen,Xue,Hao,Wu,Wei-Hua,Chen,Xue,Die,Zhang,Chen,Zhang,Chen,Die,Dai,Senying,Lai,Wang,Wei-Hua,Chen,Gao,Wei-Hua,Chen,L,Na,Gao,Sicheng,Wu,Wang,Wei-Hua,Chen,Wang,Chen,Wei-Hua,Chen,Hao,Wei-Hua,Chen,Zhang,Zhang,Wei-Hua,Chen,Lin,Zhang,Wei-Hua,Chen,Guanting,Lu,Chen,Wang,Wu,Wei-Hua,Chen,Jian,Ge,Gao,Wu,Wang,Zhang,Chen,Guanting,Lu,Wei-Hua,Chen,Gao,Huangkai,Zhang,Guanting,Lu,Maria,Wei-Hua,Chen,Daniel,Eva,L,Maria,Eva,Verónica,Chen,L,Wei-Hua,Chen,Wei-Hua,Chen,Lluch‐Senar,Chen,Marc,Wei-Hua,Chen,Gao,Kalliopi,Trachana,Wei-Hua,Chen,Wu,Wei-Hua,Chen,Yu,Xiaomeng,Ge,Yu,Weiwei,Wang,Song,Chen,Chen,Zhang,Jiang,Chen,Guanting,Wang,Lin,Wu,He,Lin,Wang,Yu,Xiaomeng,Ge,Chen,Marc,Wei-Hua,Chen,Sebastian,Wei-Hua,Chen,Eva,Yus,Wei-Hua,Chen,Puzi,Jiang,Wei-Hua,Chen,Zhang,L,Na,Gao,Wang,He,L,Hao,Puzi,Jiang,Wei-Hua,Chen,Zhang,Balakrishnan,Wei-Hua,Chen,Zhang,Chen,Zhang,Wei-Hua,Chen,Sicheng,Wu,Lai,Wei-Hua,Chen,Xue,Hao,Wu,Wei-Hua,Chen,Xue,Die,Zhang,Chen,Zhang,Chen,Die,Dai,Lai,Wang,Wei-Hua,Chen,Gao,Wei-Hua,Chen,L,Na,Gao,Wu,Wang,Wei-Hua,Chen,Wang,Chen,Wei-Hua,Chen,Hao,Wei-Hua,Chen,Zhang,Zhang,Wei-Hua,Chen,Lin,Zhang,Wei-Hua,Chen,Guanting,Lu,Chen,Wang,Wu,Wei-Hua,Chen,Jian,Ge,Gao,Wu,Wang,Zhang,Chen,Guanting,Lu,Wei-Hua,Chen,Gao,Huangkai,Zhang,Guanting,Lu,Maria,Wei-Hua,Chen,Eva,Yus,Maria,Eva,Yus,Chen,Wei-Hua,Chen,Wei-Hua,Chen,Chen,Marc,Wei-Hua,Chen,Gao,Kalliopi,Wei-Hua,Chen,Wu,Wei-Hua,Chen,Yu,Xiaomeng,Ge,Yu,Wang,Song,Chen,Chen,Zhang,Jiang,Chen,Guanting,Wang,Lin,Wu,He,Lin,Wang,Yu,Xiaomeng,Chen,Marc,Guell,Wei-Hua,Chen,Sebastian,Kuhner,Wei-Hua,Chen,Eva,Yus,Wei-Hua,Chen,Jiang,Wei-Hua,Chen,Lixin,Zhang,Gao,Yan,Wang,Li-jie,He,B,Robin,Gasser,K,Pasi,Korhonen,D,Neil,Young,Tulio,Campos,Hao,Puzi,Jiang,Wei-Hua,Chen,Yanqi,Jinglong,Zhang,Wei-Hua,Chen,Zichao,Zhang,Shaojun,Pan,Chen,Han,Cheng,Yifeng,Zhang,Yimeng,Du,Hong,Qianqian,Xiujuan,Luye,Shi,Wei-Hua,Chen,Wu,Xiushan,Yin,Wei-Hua,Chen,Vicent,Pelechano,Lingling,Mao,Xue,Dong,Hao,Shanshan,Wu,Wei-Hua,Chen,Xue,Die,Zhang,Fengguo,Chen,Zunjian,Zhang,Menglu,Chen,Yong-Qing,Die,Dai,Pengyu,Yaling,Yang,Longhao,Jia,Wang,Yanze,Chuqing,Wei-Hua,Chen,Gao,Wei-Hua,Chen,Gao,Wu,Wang,Wei-Hua,Chen,Wang,Chen,Wei-Hua,Chen,Xiaowen,Hao,Wei-Hua,Chen,Zhang,Zhang,Wei-Hua,Chen,Zhang,Wei-Hua,Chen,Guanting,Lu,Shijie,Mu,Chen,Zhiqiang,Wang,Gangbin,Jin,Wu,Qibin,Luo,Wen-Bin,Xie,Pei-Pei,Wei-Hua,Chen,Jian,Gao,Wu,Wang,Zhang,Chen,Wei-Hua,Chen,Gao,Zhang,I,John,Glass,Wei-Hua,Chen,Amparo,Latorre,Rosario,Gil,Tjerko,Kamminga,Cano,Christina,Pesavento,Daniel,Mende,Guglielmo,Roma,Andreu,Alibés,Eva,Yus,H.,L,Marco,Hennrich,Maria,Lluch-Senar,Robert,Nichols,Eva,Yus,Besray,E,Unal,Francis,O'Reilly,Lloréns‐Rico,Chen,L,Wei-Hua,Chen,Michael,R,Garavito,F,Jillian,Banfield,Brett,Baker,Andrea,Bräutigam,Mario,Stanke,Roshan,Shrestha,G,Guillaume,Barbier,M,Chad,Ternes,Wei-Hua,Chen,Luis,Serrano,Anne‐Claude,Gavin,Javier,Delgado,María,Chen,P,Ana,Vivancos,Marc,Güell,Wei-Hua,Chen,Shenghan,Gao,Kalliopi,Wei-Hua,Chen,Wu,Pablo,Minguez,Wei-Hua,Chen,Xiaomeng,Wang,Chen,Chen,Jean,Muller,Wei‐Hua,Chen,Tomas,Larsson,Xiao-Ning,Wang,Song-Nian,Ying,Zhen-Qiang,Wu,Xiao-Wei,Xue-Xia,Wang,Juliette,Meaux,de,Xiaomeng,Ge,Chen,Marc,Guell,Wei-Hua,Chen,Sebastian,Kuhner,Wei-Hua,Chen,Eva,Yus,Wei-Hua,Chen,Wei-Hua,Chen,Xue,Wei-Hua,Chen,Wang,Chen,Wei-Hua,Chen,Wei-Hua,Chen,Jian,Ge,Gao,Wang,Chen,L,Wei-Hua,Chen,Wei-Hua,Chen,Xiaomeng,Ge,Wang,Chen,Chen,Xiaomeng,Ge,Marc,Guell,Wei-Hua,Kuhner,Wei-Hua,Eva,Wei-Hua,Xue,Wei-Hua,Wang,Wei-Hua,Wei-Hua,A,Hasan,Aljohi,Ting,Wei,Qiang,Wanfei,Jian,Ge,Gao,Wang,Wei-Hua,Chen,Wei-Hua,Chen,Xiaomeng,Ge,Wang,Chen,Zhengyu,Shu,Baoyu,Tian,Chen,Xiaowei,Xianzhang,Siqi,Xiaomeng,Ge,Chen,Bo,Meng,Marc,Guell,Chen,Sebastian,Kuhner,Chen,Eva,Zheng-Bo,Xue,Dan-Dan,Fan,Jiao,Shao,Bian-Jin,Sun,Yi,Xu,Yu-Yu,Chen,Teng,Jingchao,Chen,Chen,Zhi,Liu,Zhanbing,Chengwei,Li,Fangqing,Ge,Jianmin,Xiaoyue,Suhua,Chang,Kunlin,Kai,Ye,Eric-Wubbo,Lameijer,Peer,Bork,Xing-Ming,Zhao,Xiao,Chen,Ivana,Vonkova,Georgy,Smyshlyaev,Panagiotis,Kastritis,T,Sean,Powell,Jaime,Huerta-Cepas,Noella,Silva-Martin,Nayab,Malik,Alessandro,Ori,Chen,Chen,Martin,Lercher,Haolei,Wan,Xiaomeng,Jun,Shuhui,Weihua,Chen,Songnian,Hu,Changqing,Zeng,Congying,Lv,Ronan,Bourgeois,Sira,Martínez,Marc,AH,Judith,Wodke,Doerks,Manimozhiyan,Arumugam,Konstantinos,Michalodimitrakis,Justine,Leigh-Bell,Pedro,Beltran-Alvarez,Samuel,Bader,Tobias,Maier,Takuji,Yamada,Michaela,Rode,Claire,Batisse,Alejandra,Leo-Macias,J,Matthew,Betts,Vera,Noort,van

Journal: Fungal biology and biotechnology

Volume: 8

Issue: 1

Page Number: 1-9

DOI number: 10.1186/s40694-021-00108-5

Date of Publication: 2021-02-18

Impact Factor: 2.789

Abstract: Background Sclerotium rolfsii is a potent producer of many secondary metabolites, one of which like scleroglucan is an exopolysaccharide (EPS) appreciated as a multipurpose compound applicable in many industrial fields. Results Aspartate transaminase (AAT1) catalyzes the interconversion of aspartate and α-ketoglutarate to glutamate and oxaloacetate. We selected AAT1 in the oxalate metabolic pathway as a target of CRISPR/Cas9. Disruption of AAT1 leads to the accumulation of oxalate, rather than its conversion to α-ketoglutarate (AKG). Therefore, AAT1-mutant serves to lower the pH (pH 3–4) so as to increase the production of the pH-sensitive metabolite scleroglucan to 21.03 g L−1 with a productivity of up to 0.25 g L−1·h−1. Conclusions We established a platform for gene editing that could rapidly generate and select mutants to provide a new beneficial strain of S. rolfsii as a scleroglucan hyper-producer, which is expected to reduce the cost of controlling the optimum pH condition in the fermentation industry.