论文类型：基础研究

论文编号：PMID: 26387920

第一作者：Ming Zhao, Xiaoyang Wan

通讯作者：Leilei Peng & Weibin Zhou

合写作者： Yu Li, Weibin Zhou Leilei Peng

发表刊物：Scientific Reports

收录刊物：SCI

所属单位：the University of Michigan

刊物所在地：British

学科门类：理学

一级学科：生物学

卷号：5

期号：13991

ISSN号：0068-1261

发表时间：2015-09-21

摘要：Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos with high spatial resolution. The method simultaneously images fluorescence lifetimes in 3D with multiple excitation lasers. Through quantitative analysis of triple-channel intensity and lifetime images, we demonstrated that Ca(2+) and cAMP levels of live e

发布期刊链接：https://www.ncbi.nlm.nih.gov/pubmed/26387920