研究方向：

致力于微流控生物芯片与纳微尺度生物医学技术相关研究，针对“生物”国家战略性新兴产业与世界科技前沿，探索生物医学工程和分析化学领域的前沿科学问题，研发面向单细胞分析、液体活检以及体外快速诊断的创新技术、方法和仪器。

一作及通讯作者论文

[1] Pengjie Li; Peng Chen; Fukang Qi; Jinyun Shi; Wenjie Zhu; Jiashuo Li; Peng Zhang; Han Xie; Lina Li; Mengcheng Lei; Xueqing Ren; Wenhui Wang; Liang Zhang; Xufu Xiang; Yiwei Zhang; Zhaolong Gao; Xiaojun Feng; Wei Du; Xin Liu; Limin Xia; Bi-Feng Liu; Yiwei Li. High-throughput and proteome-wide discovery of endogenous biomolecular condensates. Nature Chemistry 2024, 16 (7), 1101-1112.

[2] Xudong Zhao; Xiang Liu; Tucan Chen; Han Xie; Shunji Li; Ying Zhang; Hongwei Zhang; Yulin Cao; Wei Du; Xiaojun Feng; Xin Liu; Yiwei Li; Peng Chen; Qiubai Li; Bi-Feng Liu. Fully Integrated Centrifugal Microfluidics for Rapid Exosome Isolation, Glycan Analysis, and Point-of-Care Diagnosis. ACS Nano 2025, 19 (9), 8948-8965.

[3] Han Xie; Xubin Zhu; Kaiyu Chen; Zhilin Zhang; Jinzhi Liu; Wenhui Wang; Chao Wan; Jieqing Wang; Di Peng; Yiwei Li; Peng Chen; Bi-Feng Liu. Freeze–Thaw Imaging for Microorganism Classification Assisted with Artificial Intelligence. ACS Nano 2025, 19 (8), 8162–8175.

[4] Xin Wang; Xiaolu Cai; Chao Wan; Huijuan Yuan; Shunji Li; Yiwei Zhang; Ran Zhao; Yuxi Qin; Yiwei Li; Bi-Feng Liu; Peng Chen. Data-Driven Theoretical Modeling of Centrifugal Step Emulsification and Its Application in Comprehensive Multiscale Analysis. Advanced Science 2025, 2411459.

[5] Jie Wang; Peng Chen; Yue Dong; Han Xie; Yachao Wang; Fernando Soto; Peng Ma; Xiaojun Feng; Wei Du; Bi-Feng Liu. Designer exosomes enabling tumor targeted efficient chemo/gene/photothermal therapy. Biomaterials 2021, 276, 121056.

[6] Han Xie; Dongjuan Chen; Mengcheng Lei; Yuanyuan Liu; Xudong Zhao; Xueqing Ren; Jinyun Shi; Huijuan Yuan; Pengjie Li; Xubing Zhu; Wei Du; Xiaojun Feng; Xin Liu; Yiwei Li; Peng Chen; Bi-Feng Liu. Freeze-Thaw-Induced Patterning of Extracellular Vesicles with Artificial Intelligence for Breast Cancers Identifications. Small 2025, 21 (4), 2408871.

[7] Huijuan Yuan; Chao Wan; Xin Wang; Shunji Li; Han Xie; Chungen Qian; Wei Du; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. Programmable Gravity Self-Driven Microfluidic Chip for Point-of-Care Multiplied Immunoassays. Small 2024, 20 (21), 2310206.

[8] Shunji Li; Ying Zhang; Jingxuan Liu; Xing Wang; Chungen Qian; Jingjing Wang; Liqiang Wu; Chenxi Dai; Huijuan Yuan; Chao Wan; Jiashuo Li; Wei Du; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. Fully Integrated and High-Throughput Microfluidic System for Multiplexed Point-Of-Care Testing. Small 2024, 2401848.

[9] Huijuan Yuan; Zeyu Miao; Chao Wan; Jingjing Wang; Jinzhi Liu; Yiwei Li; Yujin Xiao; Peng Chen; Bi-Feng Liu. Recent advances in centrifugal microfluidics for point-of-care testing. Lab on a Chip 2025, 10.1039/D4LC00779D.

[10] Kezhen Yi; Zhonglin Zhang; Peng Chen; Xiaodan Xi; Xudong Zhao; Yuan Rong; Fei Long; Qian Zhang; Ying Zhang; Menglu Gao; Weihuang Liu; Bi-Feng Liu; Zhenyu Zhu; Fubing Wang. Tidal microfluidic chip-based isolation and transcriptomic profiling of plasma extracellular vesicles for clinical monitoring of high-risk patients with hepatocellular carcinoma-associated precursors. Biosensors and Bioelectronics 2025, 276, 117228.

[11] Siyu Gao; Jingjing Wang; Zeyu Miao; Xudong Zhao; Ying Zhang; Wei Du; Xiaojun Feng; Yiwei Li; Jinzhi Liu; Peng Chen; Bi-Feng Liu. Artificial intelligence enhanced microfluidic system for multiplexed point-of-care-testing of biological thiols. Talanta 2025, 287, 127619.

[12] Yilan Zhang; Fukang Qi; Peng Chen; Bi-Feng Liu; Yiwei Li. Spatially defined microenvironment for engineering organoids. Biophysics Reviews 2024, 5 (4).

[13] Yunhao Zhang; Wenjie Lv; Zixin Kang; Anxin Guo; Junming Li; Chenxi Dai; Mingyu Zhang; Siyu Gao; Shunji Li; Zeyu Miao; Sihan Chen; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. Drip-Dry Strategy Assisted Blu-Ray Disc Biosensor for Fast Point of Care Testing. Analytical Chemistry 2024, 96 (38), 15265-15276.

[14] Yujin Xiao; Mengfan Zhou; Changgen Liu; Siyu Gao; Chao Wan; Shunji Li; Chenxi Dai; Wei Du; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. Fully integrated and automated centrifugal microfluidic chip for point-of-care multiplexed molecular diagnostics. Biosensors and Bioelectronics 2024, 255, 116240.

[15] Chao Wan; Longyu Yi; Huijuan Yuan; Shunji Li; Xin Wang; Yuxiao Shu; Han Xie; Mengcheng Lei; Zeyu Miao; Wei Du; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. Atmospheric Pressure Enhanced Self-Sealing Rotation-SlipChip with Programmable Concentration Gradient Generation for Microbiological Applications. Small Methods 2024, 8 (12), 2400454.

[16] Chungen Qian; Pengjie Li; Jingjing Wang; Xianzhe Hong; Xudong Zhao; Liqiang Wu; Zeyu Miao; Wei Du; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. Centrifugo-Pneumatic Reciprocating Flowing Coupled with a Spatial Confinement Strategy for an Ultrafast Multiplexed Immunoassay. Analytical Chemistry 2024, 96 (18), 7145-7154.

[17] Zheng Pang; Shunji Li; Shangang Wang; Zonglin Cai; Shuo Zhang; Chao Wan; Jieqing Wang; Yiwei Li; Peng Chen; Bi-Feng Liu. Controlled-diffusion centrifugal microfluidic for rapid antibiotic susceptibility testing. Analytica Chimica Acta 2024, 1287, 342033.

[18] Shunji Li; Chao Wan; Yujin Xiao; Changgen Liu; Xudong Zhao; Ying Zhang; Huijuan Yuan; Liqiang Wu; Chungen Qian; Yiwei Li; Peng Chen; Bi-Feng Liu. Multiple on-line active valves based centrifugal microfluidics for dynamic solid-phase enrichment and purification of viral nucleic acid. Lab on a Chip 2024, 24 (12), 3158-3168.

[19] Song Guo; Han Xie; Xudong Zhao; Honghao He; Xiaojun Feng; Yiwei Li; Bi-Feng Liu; Peng Chen. All-in-one detection of breast cancer-derived exosomal miRNA on a pen-based paper chip. Analyst 2024, 149 (4), 1250-1261, 10.1039/D3AN02032K.

[20] Chungen Qian; Chao Wan; Shunji Li; Yujin Xiao; Huijuan Yuan; Siyu Gao; Liqiang Wu; Mengfan Zhou; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. On-Line Dual-Active Valves Based Centrifugal Microfluidic Chip for Fully Automated Point-of-Care Immunoassay. Analytical Chemistry 2023, 95 (33), 12521-12531.

[21] Chungen Qian; Jiashuo Li; Zheng Pang; Han Xie; Chao Wan; Shunji Li; Xin Wang; Yujin Xiao; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. Hand-powered centrifugal micropipette-tip with distance-based quantification for on-site testing of SARS-CoV-2 virus. Talanta 2023, 258, 124466.

[22] Shunji Li; Chao Wan; Bangfeng Wang; Dongjuan Chen; Wenyi Zeng; Xianzhe Hong; Lina Li; Zheng Pang; Wei Du; Xiaojun Feng; Peng Chen; Yiwei Li; Bi-Feng Liu. Handyfuge Microfluidic for On-Site Antibiotic Susceptibility Testing. Analytical Chemistry 2023, 95 (14), 6145-6155.

[23] Qi Huang; Wenjing Xiao; Peng Chen; Hui Xia; Sufei Wang; Yice Sun; Qi Tan; Xueyun Tan; Kaimin Mao; Han Xie; Ping Luo; Limin Duan; Daquan Meng; Yanling Ma; Zilin Zhao; Fen Wang; Jianchu Zhang; Bi-Feng Liu; Yang Jin. Nanopore membrane chip-based isolation method for metabolomic analysis of plasma small extracellular vesicles from COVID-19 survivors. Biosensors and Bioelectronics 2023, 227, 115152.

[24] Mengfan Zhou; Huiying Su; Bangfeng Wang; Chao Wan; Wei Du; Peng Chen; Xiaojun Feng; Bi-Feng Liu. A magnet-actuated microfluidic array chip for high-throughput pretreatment and amplification and detection of multiple pathogens. Analyst 2022, 147 (11), 2433-2441, 10.1039/D2AN00430E.

[25] Wenyi Zeng; Peng Chen; Shunji Li; Qiuyue Sha; Pengjie Li; Xuemei Zeng; Xiaojun Feng; Wei Du; Bi-Feng Liu. Hand-powered vacuum-driven microfluidic gradient generator for high-throughput antimicrobial susceptibility testing. Biosensors and Bioelectronics 2022, 205, 114100.

[26] Huijuan Yuan; Peng Chen; Chao Wan; Yiwei Li; Bi-Feng Liu. Merging microfluidics with luminescence immunoassays for urgent point-of-care diagnostics of COVID-19. TrAC Trends in Analytical Chemistry 2022, 157, 116814.

[27] Yujin Xiao; Shunji Li; Zheng Pang; Chao Wan; Lina Li; Huijuan Yuan; Xianzhe Hong; Wei Du; Xiaojun Feng; Yiwei Li; Peng Chen; Bi-Feng Liu. Multi-reagents dispensing centrifugal microfluidics for point-of-care testing. Biosensors and Bioelectronics 2022, 206, 114130.

[28] Xin Wang; Xian-Zhe Hong; Yi-Wei Li; Ying Li; Jie Wang; Peng Chen; Bi-Feng Liu. Microfluidics-based strategies for molecular diagnostics of infectious diseases. Military Medical Research 2022, 9 (1), 11.

[29] Yiran Guo; Peng Chen; Zhaolong Gao; Yiwei Li; Shunji Li; Xiaojun Feng; Bi-Feng Liu. A time-coded multi-concentration microfluidic chemical waveform generator for high-throughput probing suspension single-cell signaling. Chinese Chemical Letters 2022, 33 (6), 3091-3096.

[30] Chungen Qian; Yujin Xiao; Jie Wang; Yiwei Li; Shunji Li; Bo Wei; Wei Du; Xiaojun Feng; Peng Chen; Bi-Feng Liu. Rapid exosomes concentration and in situ detection of exosomal microRNA on agarose-based microfluidic chip. Sensors and Actuators B: Chemical 2021, 333, 129559.

[31] Peng Chen; Chen Chen; Huiying Su; Mengfan Zhou; Shunji Li; Wei Du; Xiaojun Feng; Bi-Feng Liu. Integrated and finger-actuated microfluidic chip for point-of-care testing of multiple pathogens. Talanta 2021, 224, 121844.

[32] Yiran Guo; Zhaolong Gao; Yinan Liu; Shunji Li; Jinchi Zhu; Peng Chen; Bi-Feng Liu. Multichannel Synchronous Hydrodynamic Gating Coupling with Concentration Gradient Generator for High-Throughput Probing Dynamic Signaling of Single Cells. Analytical Chemistry 2020, 92 (17), 12062-12070.

[33] Peng Chen; Shunji Li; Yiran Guo; Xuemei Zeng; Bi-Feng Liu. A review on microfluidics manipulation of the extracellular chemical microenvironment and its emerging application to cell analysis. Analytica Chimica Acta 2020, 1125, 94-113.

[34] Peng Chen; Shuangqian Yan; Jie Wang; Yiran Guo; Yue Dong; Xiaojun Feng; Xuemei Zeng; Yiwei Li; Wei Du; Bi-Feng Liu. Dynamic Microfluidic Cytometry for Single-Cell Cellomics: High-Throughput Probing Single-Cell-Resolution Signaling. Analytical Chemistry 2019, 91 (2), 1619-1626.

[35] Peng Chen; Dongjuan Chen; Shunji Li; Xiaowen Ou; Bi-Feng Liu. Microfluidics towards single cell resolution protein analysis. TrAC Trends in Analytical Chemistry 2019, 117, 2-12.

[36] Peng Chen; Chen Chen; Yuhong Liu; Wei Du; Xiaojun Feng; Bi-Feng Liu. Fully integrated nucleic acid pretreatment, amplification, and detection on a paper chip for identifying EGFR mutations in lung cancer cells. Sensors and Actuators B: Chemical 2019, 283, 472-477.

[37] Peng Chen; Xiaojun Feng; Shuangqian Yan; Yiran Guo; Jie Wang; Yiwei Li; Dongjuan Chen; Wei Du; Bi-Feng Liu. Precision guided microfluidic chemical perfusion strategy for cell dynamic imaging. Sensors and Actuators B: Chemical 2018, 263, 281-288.

[38] Shuangqian Yan; Peng Chen; Xuemei Zeng; Xian Zhang; Yiwei Li; Yun Xia; Jie Wang; Xiaofang Dai; Xiaojun Feng; Wei Du; Bi-Feng Liu. Integrated Multifunctional Electrochemistry Microchip for Highly Efficient Capture, Release, Lysis, and Analysis of Circulating Tumor Cells. Analytical Chemistry 2017, 89 (22), 12039-12044.

[39] Peng Chen; Yiran Guo; Jie Wang; Wei Du; Xiaojun Feng; Bi-Feng Liu. A localized chemical pulse generator for the development of a microfluidic cell-based biosensor. Sensors and Actuators B: Chemical 2017, 251, 112-119.

[40] Peng Chen; Yiran Guo; Xiaojun Feng; Shuangqian Yan; Jie Wang; Yiwei Li; Wei Du; Bi-Feng Liu. Microfluidic Chemical Function Generator for Probing Dynamic Cell Signaling. Analytical Chemistry 2017, 89 (17), 9209-9217.

[41] Peng Chen; Xiaojun Feng; Dongjuan Chen; Chao Liu; Wei Du; Bi-Feng Liu. Investigating intercellular calcium waves by microfluidic gated pinched-flow. Sensors and Actuators B: Chemical 2016, 234, 583-592.

[42] Peng Chen; Pu Chen; Xiaojun Feng; Wei Du; Bi-Feng Liu. Analysis of intercellular communication by flexible hydrodynamic gating on a microfluidic chip. Analytical and Bioanalytical Chemistry 2013, 405 (1), 307-314.