论文类型：期刊论文

第一作者：Yang Li,Ming-liang Ma,Qing Lei,Feng Wang,Wei Hong

通讯作者：Hongping Wei,Ziyong Sun,Xionglin Fan,Sheng-ce Tao

合写作者：He-wei Jiang,Yun-xiao Zheng,Xue-ning Wang,Jiaoxiang Wu,Ming-liang Ma,Bo Zhang,Hong Chen,Caizheng Yu,Jun-biao Xue,Hai-nan Zhang,Huan Qi,Shu-juan Guo,Yandi Zhang,Xiaosong Lin,Zongjie Yao,Huiming Sheng,Ziyong Sun

发表刊物：Cell Reports

所属单位：华中科技大学

刊物所在地：United Kingdom

学科门类：医学

一级学科：基础医学

文献类型：J

卷号：Volume 34

期号：13

页面范围：108915

ISSN号：0105-4538

关键字：COVID-19; SARS-CoV-2; Spike protein; linear epitope landscape; neutralizing antibodies.

DOI码：10.1016/j.celrep.2021.108915

发表时间：2021-03-12

影响因子：9.423

教研室：华中科技大学基础医学院病原生物学系医学微生物学

摘要：To fully decipher the immunogenicity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein, it is essential to assess which part is highly immunogenic in a systematic way. We generate a linear epitope landscape of the Spike protein by analyzing the serum immunoglobulin G (IgG) response of 1,051 coronavirus disease 2019 (COVID-19) patients with a peptide microarray. We reveal two regions rich in linear epitopes, i.e., C-terminal domain (CTD) and a region close to the S2' cleavage site and fusion peptide. Unexpectedly, we find that the receptor binding domain (RBD) lacks linear epitope. We reveal that the number of responsive peptides is highly variable among patients and correlates with disease severity. Some peptides are moderately associated with severity and clinical outcome. By immunizing mice, we obtain linear-epitope-specific antibodies; however, no significant neutralizing activity against the authentic virus is observed for these antibodies. This landscape will facilitate our understanding of SARS-CoV-2-specific humoral responses and might be useful for vaccine refinement.

备注：Y.Li,M. Ma,Q.Lei, etal. Linear epitope landscape of SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients. Cell reports, 2021,34(13): 108915.

发布期刊链接：https://www.sciencedirect.com/science/article/pii/S2211124721002291?via%3Dihub