论文类型：期刊论文

第一作者：Yang Li，Zhaowei Xu，Qing Lei，Dan-yun Lai，Hongyan Hou

通讯作者：Feng Wang,Xionglin Fan,Sheng-ce Tao

合写作者：He-wei Jiang,Yun-xiao Zheng,Xue-ning Wang,Jiaoxiang Wu,Ming-liang Ma,Bo Zhang,Hong Chen,Caizheng Yu,Jun-biao Xue,Hai-nan Zhang,Huan Qi,Shu-juan Guo ,Yandi Zhang,Xiaosong Lin,Zongjie Yao,Huiming Sheng,Ziyong Sun

发表刊物：Cell Reports

所属单位：华中科技大学

学科门类：医学

一级学科：基础医学

文献类型：J

卷号：36

期号：2

ISSN号：2211-1247

关键字：COVID-19; SARS-CoV-2; humoral immunity; immune response; non-structural/accessory proteins; proteome microarray

DOI码：10.1016/j.celrep.2021.109391

发表时间：2021-06-26

影响因子：9.423

教研室：华中科技大学同济医学院基础医学院病原生物学

摘要：The immunogenicity of the SARS-CoV-2 proteome is largely unknown, especially for non-structural proteins and accessory proteins. In this study, we collect 2,360 COVID-19 sera and 601 control sera. We analyze these sera on a protein microarray with 20 proteins of SARS-CoV-2, building an antibody response landscape for immunoglobulin (Ig)G and IgM. Non-structural proteins and accessory proteins NSP1, NSP7, NSP8, RdRp, ORF3b, and ORF9b elicit prevalent IgG responses. The IgG patterns and dynamics of non-structural/accessory proteins are different from those of the S and N proteins. The IgG responses against these six proteins are associated with disease severity and clinical outcome, and they decline sharply about 20 days after symptom onset. In non-survivors, a sharp decrease of IgG antibodies against S1 and N proteins before death is observed. The global antibody responses to non-structural/accessory proteins revealed here may facilitate a deeper understanding of SARS-CoV-2 immunology.