Indexed by: Unit Twenty Basic Research

Document Code: PMID: 26387920

First Author: Ming Zhao, Xiaoyang Wan

Correspondence Author: Leilei Peng & Weibin Zhou

Co-author: Yu Li, Weibin Zhou Leilei Peng

Journal: Scientific Reports

Included Journals: SCI

Affiliation of Author(s): the University of Michigan

Place of Publication: British

Discipline: Science

First-Level Discipline: Biology

Volume: 5

Issue: 13991

ISSN No.: 0068-1261

Date of Publication: 2015-09-21

Abstract: Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos with high spatial resolution. The method simultaneously images fluorescence lifetimes in 3D with multiple excitation lasers. Through quantitative analysis of triple-channel intensity and lifetime images, we demonstrated that Ca(2+) and cAMP levels of live e

Links to published journals: https://www.ncbi.nlm.nih.gov/pubmed/26387920