Indexed by:Journal paper First Author:Huimin Wang,Jintao Cai Correspondence Author:王涛 Journal:Biosensors and Bioelectronics Included Journals:SCI Affiliation of Author(s):华中科技大学 Place of Publication:美国 Discipline:Engineering First-Level Discipline:Biomedical Engineering Funded by:国基金 Document Type:J Volume:295 Page Number:116295-1-116295-7 Key Words:Hyperbolic metamaterial Functionalized gold nanoparticle Plasmonic biosensor Carcinoembryonic antigen Date of Publication:2024-04-12 Impact Factor:12.0 Teaching and Research Group:武汉光电国家研究中心 Abstract:Hyperbolic metamaterial (HMM) biosensors based on metals have superior performance in comparison with conventional plasmonic biosensors in the detection of low concentrations of molecules. In this study, a nanorod HMM (NHMM) biosensor based on refractive index changes for carcinoembryonic antigen (CEA) detection is developed using secondary antibody modified gold nanoparticle (AuNP-Ab2) nanocomposites as signal amplification element for the first time. Numerical analysis based on finite element method is conducted to simulate the perturbation of the electric field of bulk plasmon polariton (BPP) supported by a NHMM in the presence of a AuNP. The simulation reveals an enhancement of the localized electric field, which arises from the resonant coupling of BPP to the localized surface plasmon resonance supported by AuNPs and is beneficial for the detection of changes of the refractive index. Furthermore, the AuNP-Ab2 nanocomposites-based NHMM (AuNP/Ab2-NHMM) biosensor enables CEA detection in the visible and near-infrared regions simultaneously. The highly sensitive detection of CEA with a wide linear range of 1–500 ng/mL is achieved in the near-infrared region. The detectable concentration of the AuNP/Ab2-NHMM biosensor has a 50-fold decrease in comparison with a NHMM biosensor. A low detection limit of 0.25 ng/mL (1.25 pM) is estimated when considering a noise level of 0.05 nm as the minimum detectable wavelength shift. The proposed method achieves high sensitivity and good reproducibility for CEA detection, which makes it a novel and viable approach for biomedical research and early clinical diagnostics. Links to published journals:https://www.sciencedirect.com/science/article/pii/S0956566324003002?via%3Dihub